Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammation

Background Asthma is the most common allergic disease characterized by an inflammatory response in the airways. Mechanismly, urban particulate matter (PM) is the most widely air pollutant associated with increased asthma morbidity and airway inflammation. Current research found that vitamin D is an essential vitamin with anti-inflammatory, antioxidant and other medical efficacy. Inadequate or deficient vitamin D often leads to the pathogenesis and stability of asthma. NGF exacerbates airway inflammation in asthma by promoting smooth muscle cell proliferation and inducing the Th2 immune response. Activation of the Nrf2/HO-1 signaling pathway can exert a protective effect on the inflammatory response in bronchial asthma. However, the specific mechanism of this pathway in PM-involved asthmatic airway smooth muscle cells remains unclear. Methods Mice were sensitized and challenged with Ovalbumin (OVA) to establish an asthma model. They were then exposed to either PM, vitamin D or a combination of both, and inflammatory responses were observed. Including, acetylcholine stimulation at different concentrations measured airway hyperresponsiveness in mice. Bronchoalveolar lavage fluid (BALF) and serum were collected for TNF-α, IL-1β, IL-6, and Nerve growth factor (NGF) analysis. Additionally, lung tissues underwent histopathological examination to observe alveolar structure and inflammatory cell infiltration. Specific ELISA kits were utilized to determine the levels of the inflammatory factors TNF-α, IL-1β, IL-6, and Nerve growth factor (NGF). Nrf2/HO-1 signaling pathways were examined by western blot analysis. Meanwhile, we constructed a cell system with low HO-1 expression by lentiviral transfection of airway smooth muscle cells. The changes of Nrf2, HO-1, and NGF were observed after the treatment of OVA, PM, and Vit D were given. Results The in vivo results showed that vitamin D significantly alleviated pathological changes in lung tissue of PM-exposed mice models. Mechanismly, vitamin D decreased substantial inflammatory cell infiltration in lung tissue, as well as the number of inflammatory cells in BALF. Furthermore, vitamin D reduced the heightened inflammatory factors including of TNF-α, IL-1β, IL-6, and NGF caused by PM exposure, and triggered the activity of nucleus Nrf2 and HO-1 in PM-exposed asthmatic mice. Notably, knockdown HO-1 weakens the Vitamin D- mediated inhibition to pollution toxicity in asthma. Importantly, in vitro experiments on OVA-stimulated mice airway smooth muscle cells, the results showed that OVA and PM, respectively, reduced Nrf2/HO-1 and increased NGF’s expression, while vitamin D reversed the process. And in the HO-1 knockdown cell line of Lenti-si-HO-1 ASMCs, OVA and PM reduced Nrf2’s expression, while HO-1 and NGF’s expression were unchanged. Conclusions The above results demastrate that vitamin D downregulated the inflammatory response and the expression of NGF by regulating the Nrf2/HO-1 signaling pathways in airway smooth muscle cells, thereby showing potent anti-inflammatory activity in asthma. Supplementary Information The online version contains supplementary material available at 10.1186/s12890-024-02869-2.


Background
Asthma is a chronic inflammatory airways disease with marked heterogeneity and complex pathophysiological manifestations.Currently, there are at least 300 million asthma cases worldwide, and the prevalence is increasing yearly, with an additional one-third of patients expected by 2025 [1].Asthma is mainly caused by genetic susceptibility and environmental exposure, with a growing number of cases attributed to airborne particles each year.Of note, the International Agency for Research on Cancer (IARC) has ranked airborne particulate matter (PM) as a class I carcinogen [2].In addition, many studies have demonstrated that asthma cases are exacerbated by geographic exposure to airborne particulate matter [3][4][5][6].
Vitamin D3 is a fat-soluble vitamin with various pharmaceutical properties, including anti-inflammatory, immunomodulatory activities, the promotion of barrier function, wound healing and hair growth, and anticancer [7,8].Vitamin D supplementation modulates the host immune response to viruses [9].Vitamin D levels in peripheral blood are low in patients with systemic sclerosis [10].Importantly, vitamin D deficiency has been linked to the increase in asthma incidence over the past 20 years [11][12][13].Extensive epidemiological studies have shown that vitamin D supplementation can enhance the antiviral response of respiratory epithelial cells [14] and inhibit IL-17 A secretion in the peripheral blood of severe asthma patients [15,16].Additionally, low concentrations of vitamin D metabolite 1α, 25-hydroxyvitamin D3, were associated with an increased risk of childhood acute asthma exacerbations [17].Of note, Jolliffe reported that vitamin D supplementation significantly reduced the incidence of acute exacerbations in asthma patients treated with systemic corticosteroids [18].A study in the United States showed that vitamin D-deficient children living near major roads, high in PM exposure, were five times more likely to develop asthma than those who were vitamin D-sufficient, and living in the same areas [19].This study demonstrated that vitamin D has antioxidant effects and that traffic-related air pollution contributed to asthma morbidity.Vitamin D deficiency exacerbates airway oxidative stress induced by air pollutants, leading to an increase in severe asthma exacerbations in children.
Multiple signaling pathways are known to be involved in the pathophysiology of asthma.For example, Nrf2 has been reported to be a crucial redox-sensitive transcription factor by redox homeostasis cellular maintenance through the regulation of numerous downstream antioxidants such as heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase quinone 1 (NQO1), and superoxide dismutase (SOD) [20].Of note, HO-1 is a rate-limiting enzyme in heme degradation [21], that regulates redox reactions and has cytoprotective effects against oxidative stressinduced cellular damage.HO-1's expression is induced in the airways of asthmatic patients and has been shown to slow down allergic airway inflammation [22].Specifically, the induction of HO-1 expression reduces the production of reactive oxygen species (ROS) in the airways and alleviates IL-13-induced goblet cell proliferation, finally preventing asthma development [23].Therefore, targeting the Nrf2/HO-1 signaling pathway may be an effective strategy for asthma prevention.
It has been shown that vitamin D prevents the development of asthma linked to traffic-related air pollution exposure.Specifically, an earlier study has shown that prenatal and postnatal vitamin D supplementation significantly attenuated lung inflammation and differentiation of Th2 in asthma linked to PM exposure [12].This data suggests that vitamin D supplementation reduces children's susceptibility to the adverse effects of PM.However, the molecular mechanisms of vitamin D in PMexposed asthma are unclear.In this study, we aimed to evaluate the effects of vitamin D on OVA-induced allergic asthma in PM-exposed mice and further investigate its underlying molecular mechanisms in vitro and in vivo.

Reagents and antibodies
The Ovalbumin (OVA), Aluminum hydroxide, and TEMED were purchased from Sigma-Aldrich.The Eastep™ Total RNA Extraction Kit and GoTaq® qPCR Master Mix kit were obtained from Promega.The Strong RIPA lysate was purchased from Boster.The protein phosphatase inhibitor complex and protease inhibitors were purchased from Roche.The BCA protein quantitative kit, cDNA synthesis kit, and TRIzol Total RNA Extraction Kit were purchased from Tiangen.The ECL chemiluminescence detection kit was purchased from Advansta.The Multicolor Prestained Protein Ladder was purchased from Thermo Fisher Scientific.The PVDF membrane was purchased from EMD Millipore (Billerica, MA).The 4×loading buffer (containing DTT) was purchased from Solarbio.The Cell Proliferation Assay (CellTiter 96® AQ ueous One Solution, Cat.G3582) was purchased from Promega.The Super-PAGE™ Bis-Tris Gels and nucleoprotein extraction kit were purchased from Shanghai Epizyme Biomedical Technology.Recombinant mouse TNFα (Cat.50,349-MNAE) protein was purchased from Sino Biological.

Animal and mouse model
Wild-type BALB/c mice (female, 6-8 weeks) used in this study were obtained from the Xiamen University Laboratory Animal Center (XMULAC).All mice were maintained under specific pathogen-free (SPF) conditions at the XMULAC.Experiments on mice were approved by the Institutional Animal Care and Use Committee, and were in strict accordance with good animal practice as defined by the XMULAC.Mice were randomly divided into four groups, the control group (Control), the asthma group (Asthma), the PM-treatment asthma group (Asthma + PM), and the vitamin D and PM-treatment asthma group (Asthma + PM + Vit D).The asthma modeling and the treatment with the particulate matter were established based on earlier reports [24,25], and adapted in line with our experiments.On days 0, 7, 14, and 21 mice received an intraperitoneal (i.p.) injection with 100 µl of sensitization liquid (100 µg OVA and 2 mg aluminum hydroxide were dispersed in 100 µl of normal saline).On days 28, 29, and 30 mice were challenged with 1% OVA stimulated by nebulization.The PM-treatment and the vitamin D and PM-treatment asthma groups were administered intranasally 20 µl PM solution (100 µg PM dissolved in 20 µl saline solution) 30 min prior to being challenged.In contrast, the asthma group was given 40 µl of normal saline.The vitamin D and PMtreatment asthma group was given 300 µl of vitamin D solution (100 ng vitamin D dissolved in ethanol, and further diluted to 0.9% by 300 µl PBS) [26] by gavage on 21-30 days.All the mice were anesthetized through the nose with isoflurane before dripping.Meanwhile, the control group was administered normal saline.Mice were sacrificed by euthanized 24 h after the last nasal drip (Fig. 1a).

Lung function measurement
The mice airway reponsiveness was assessed at 24 h after the last OVA challenge using the FlexiVent system (SCIREQ Inc., Montreal, QC, Canada).After mice were anaesthetised with 60 mg/kg pentobarbital sodium (Sigma-Aldrich), they were tracheotomised and endotracheally intubated.Airway responsiveness was then assessed by measuring changes in pulmonary resistance after intubation.Changes in lung resistance were assessed after continuous exposure to increasing doses of methacholine (Sigma-Aldrich) (0, 6, 12, 24 and 48 mg/ml) in a sterile incubator.Airway resistance (Rrs) and compliance (Crs) values were recorded to analyse airway hyperresponsiveness (AHR).

Flow cytometry of lung tissue
We sacrifice the mice at 24 h after the last OVA challenge.Then we harvest the lung tissue and collect the lymphocyte through collagenase digesting, grind, and 70%/42% percoll separation.Then 1ug/ml Golgi Stop was used for blocking cytokine secretion.After 5 h, we collected the cells for surface markers FACS staining at a ratio of 1:200 (anti-CD4, anti-CD8) for 15 min.Fix Buffer/Permeabilization Buffer with 1:3 ratio was added at 4℃ for 30 min.Finally, we stained Intracellular and intranuclear cytokine using antibodies at a ratio of 1:100 (anti-IFNg, anti-IL4, anti-CD4 and anti-CD8) for 15 min.The samples were checked with a BD LSRFortessa™ flow cytometer (BD Biosciences) and analyzed with Flow Jo software (version V10).

Cell culture and lentivirus intervention
Mouse airway smooth muscle cells (ASMCs, Cat.No. iCell-m002, iCell Bioscience Inc.) were seeded in 24-well plates at 1 × 10 5 cells per well and cultured with medium supplemented with 10% fetal bovine serum.Once 80% cell fusion was reached, the cells were starved for 24 h with a serum-free medium containing 0.1% FBS.The goal was to have the cells in each group simultaneously in the G0 phase.Vitamin D was added into the well at a concentration of 10 − 7 nmol/L at 2 h before adding 100 µg/ml OVA and/or 100 ug/ml PM.Subsequently, either 100 µg/ ml OVA, 100 µg/ml PM, or both were added.After 24 h, the cell supernatant was collected.The cells were washed three times and collected for subsequent experiments.The optimal vitamin D action time and concentration were shown in Supplementary material.
HO-1-targeted short hairpin RNAs were then designed and synthesized by Genechem Co. Ltd (Shanghai, China).Details about lentivirus construction and pre-test are shown in the Supplementary material.ASMCs were infected with the lentivirus expressing a HO-1 shRNA (Lenti-si-HO-1).The optimal infection efficiency of HO-1 sh-RNA was shown in Supplementary Fig. 2 and Supplementary Fig. 3.
A cell suspension with a density of 3-5 × 10 4 cells/ml was prepared using a complete medium and seeded in 6-well plates.Cells were incubated for 16-24 h at 37 °C until the cell confluence reached 20-30%; then, the infection reagent HiTransG A and 200 µl of 1 × 108 TU/ml of adenovirus were added to the cells.After 16 h of incubation at 37 °C, the medium was replaced with a complete medium, and the culture was continued.After 72 h of infection, cells were observed for infection efficiency, and the best 1-well cells were selected for passaging.Puromycin at 2 ng/ml was added to the passages for positive screening (Fig. 4e).Importantly, Lenti-si-HO-1 ASMCs were treated as ASMC.

Experimental statistical analysis
Statistical analysis was performed with GraphPad Prism 5. One-way analysis of variance (ANOVA) with Tukey's multiple comparison test was performed to compare more than two test groups, as appropriate.A p-value of less than 0.05 was considered statistically significant.

Vitamin D ameliorated PM-exposed lung histopathological inflammation in asthmatic mice
PM-treated asthmatic mice showed a significant increase in airways resistance (Rrs), and a significant decrease in airways compliance (Crs) compared to asthmatic mice treated with methacholine at a concentration of 48 mg/ ml (Fig. 1b).While, the combination of vitamin D and PM treatment significantly decreased respiratory resistance and significantly increased respiratory compliance in asthmatic mice compared to PM-treated asthmatic mice treated with methacholine at a concentration of 48 mg/ ml (Fig. 1a).Lung histopathological staining showed that the number of inflammatory cells infiltrating the airways in both the asthma and the PM-treated asthma groups was significantly higher than in the control group.At the same time, vitamin D treatment significantly reduced the number of inflammatory cells (Fig. 1c, d).In addition, the number of inflammatory cells in BALF in the asthma group was significantly higher than in the control group (especially eosinophils).With PM treatment, the number of inflammatory cells in BALF increased.However, the number of inflammatory cells was significantly reduced in PM-treated asthmatic mice after treated with vitamin D (Fig. 1e).

Vitamin D attenuates lung inflammation in PM-exposed asthmatic mice
NGF exacerbates airway inflammation in asthma by promoting smooth muscle cell proliferation and inducing the Th2 immune response [27,28].To further evaluate the anti-inflammatory effect of vitamin D on PM-treated asthma mice, CD4 + cells in lung tissue were extracted, and the ratio of cells for Th1 and Th2 was detected.Additionally, the expression levels of inflammatory factors in BALF and serum was detected.Compared with the control group, the proportion of Th2/Th1 cells in the asthma group increased, and the rise was more significant under PM treatment.However, following vitamin D administration, the proportion of Th2/Th1 cells decreased (Fig. 2a,  2b).An ELISA kit was used to detect the levels of TNF-α, IL-1β, and IL-6 in BALF neutralization and serum.The results showed that in serum, TNF-α expression in the asthma group was significantly higher than in the control group and more pronounced under PM treatment.Of note, while TNF-α expression decreased significantly following vitamin D administration, this phenomenon was not observed in BALF (Fig. 2c).In serum and BALF, IL-1β expression increased significantly in the asthma group compared with the control group.Interestingly, while the increase was more significant under PM treatment, after vitamin D administration, IL-1β expression decreased significantly (Fig. 2c).In serum and BALF, IL-6 expression in the asthma group increased but was not significant compared to the control group (P > 0.5).On the contrary, IL-6 expression increased significantly under PM treatment, and IL-1β expression decreased significantly after the administration of vitamin D (Fig. 2c).

Vitamin D activates the PM-exposed Nrf2/HO-1 signaling pathway
Activation of the Nrf2/HO-1 signaling pathway can exert a protective effect on the inflammatory response in bronchial asthma [29].So, the effects of vitamin D on Nrf2/HO-1 expression in the asthma mouse model were further evaluated.qPCR results showed that Nrf2 and HO-1 gene expression levels in the lung tissue of the Asthma group were significantly lower compared to the Control group.Additionally, in the Asthma + PM group, the gene expression levels were significantly lower than in the Asthma group.In contrast, the gene expression levels in the Asthma + PM + Vit D group were higher than in the Asthma group and significantly higher than in the Asthma + PM group.Furthermore, the expression of NGF gene was significantly up-regulated in both the Asthma and the Asthma + PM group at first.Of note, NGF expression was significantly decreased after vitamin D administration (Fig. 3a).WB and ELISA were used to measure the expression of Nrf2/HO-1 and NGF proteins in lung tissue, respectively.The results showed that Nrf2/HO-1's expression decreased and NGF's expression increased in the Asthma group, while vitamin D administration restored the gene expression levels (Fig. 3b, 3c,  3g).This indicates that vitamin D may activate the Nrf2/ HO-1 signaling pathway in a PM-exposed asthma model, thereby reducing NGF's expression.
HO-1 is a rate-limiting enzyme in heme degradation, that regulates redox reactions and has cytoprotective effects against oxidative stress-induced cellular damage.Since NO-producing iNOS is an important marker for oxidative stress pathway activation particularly induced by pollution exposure and allergic responses [30].Therefore detecting the expression of iNOS in response to vitamin D or and PM can flank the activation of the HO-1 pathway.Since, iNOS is expressed in lung airway epithelial cells and macrophages [31], we performed qPCR and WB to detect iNOS expression in lung tissues.The results showed that iNOS shares the same expression trend with NGF and has this exact opposite expression trend with Nrf2 and HO-1 (Fig. 3d-3f ).This suggests that oxidative stress occurs in lung tissues when HO-1 is lowly expressed, that PM exposure significantly increases this oxidative stress, and that the addition of vitamin D significantly mitigates the oxidative stress.

Vitamin D activates the Nrf2/HO-1 signaling pathway in PM-exposed ASMCs
In vivo experiments preliminarily indicated that vitamin D might activate the Nrf2/HO-1 signaling pathway in the PM-exposed asthma model, thereby reducing NGF's expression.To prove the protective effect of the vitamin D-mediated Nrf2/HO-1 pathway on asthma, we performed in vitro experiments.Specifically, 10 − 7 mol/L vitamin D, 100 µg/ml OVA, and 100 µg/ml PM were co-incubated with ASMCs or Lenti-si-HO-1 ASMCs for 24 h, and then Nrf2/HO-1 expression levels were detected.Furthermore, we tested different vitamin D concentrations on ASMCs, and the optimal concentration and time of action were 10 − 7 mol/l and 24 h, respectively (shown in Supplementary Fig. 1).The results showed that OVA and PM, respectively, reduced Nrf2/ HO-1 and increased NGF's expression, while vitamin D reversed the process.In the HO-1 knockdown cell line of Lenti-si-HO-1 ASMCs, OVA and PM reduced Nrf2's expression, while HO-1 and NGF's expression were unchanged.These results suggest that vitamin Dreverses Nrf2's expression, but does not affect HO-1 and NGF's expression (Fig. 4).

Discussion
This study shows that vitamin D effectively attenuates airway inflammation in PM-exposed asthmatic mice.Concretely, it ameliorates alveolar wall thickening, decreases mucus secretion without affecting the loss of alveolar structure, and reduces inflammatory cell infiltration.Of note, our in vivo, and in vitro molecular mechanism experiments have shown that vitamin D activates the Nrf2-HO-1 pathway and inhibits NGF's expression.These results suggest that vitamin D supplementation in asthmatic mice activates the Nrf2-HO-1 pathway and suppresses NGF expression, thereby alleviating airway inflammation by reversing damage caused by PM exposure.
Recently, there has been increasing interest in the therapeutic use of vitamin D in asthma, and numerous reports have emerged on the adjuvant therapeutic effects of vitamin D supplementation in asthma.Based on multiple randomized, double-blind, parallel, placebocontrolled clinical trials, participants were randomly assigned to studies in which they took either a daily placebo capsule or a daily dose of vitamin D3 (e.g., 4,000 IU).The data from the Meta-analysis showed that vitamin D supplementation reduced the rate of acute asthma exacerbations requiring systemic corticosteroid therapy, while the duration of asthma exacerbations remained unchanged [18,32].Brustad et al. showed that supplementation with standard or high doses of vitamin D during pregnancy might have a protective effect against the onset of wheezing in children aged three years; however, this protective effect was not associated with the risk of developing asthma in six years old children [33].In contrast, Lu et al. indicated that adequate vitamin D supplementation during pregnancy in mothers with asthma might provide good protection for their offspring by the age of three years [34].Nevertheless, no consensus has been reached to date regarding the positive effects of vitamin D interventions on asthma.Specifically, other studies on diet and asthma showed that a higher vitamin D intake reduced the risk of wheezing in children [35,36].Remarkably, in their review, Garcia-Larsen et al. indicated that the protective effects of dietary antioxidants in asthma were more pronounced in childhood and were proportional to the duration of antioxidant use [37].Discrepancies in the findings of the above observational studies are perhaps due to insufficient sample size and differences in patients' baseline data.
Regardless of the controversies arising in epidemiological studies, more scholars favor the protective effect of vitamin D on asthma [32,38].Of note, numerous basic studies consistently show that vitamin D has anti-inflammatory and antioxidant protective effects against asthma.Specifically, it has been shown that vitamin D alters the development of immune cells, prolongs the survival and migration of eosinophils, and inhibits fibroblast secretion of fibrogenic mediators [39].Vitamin D has also been proven to stabilize alveolar type II cell DNA synthesis [40,41] and to modify alveolarization [42].Other studies have shown that the active metabolite of vitamin D is protective in asthmatic rodents [43,44].Based on these findings, more conclusive evidence is needed to understand the exact mechanisms of vitamin D's anti-inflammatory and antioxidant activities.Therefore, hopefully, more in-depth basic studies will be conducted.
Epidemiological studies have shown that children with vitamin D deficiency are susceptible to exposure to traffic-related particulate pollutants.Bolcas et al. [12] reported that maintaining the normal level of vitamin D during the early life of an asthma mouse model has several effects.Specifically, it can reduce the number of Th1/ Th17 cells in the lung, inhibit airway hyperresponsiveness, and significantly reduce the aggravation of allergic asthma related to diesel exhaust particulate (DEP) exposure.The authors showed that vitamin D might effectively prevent asthma development in people exposed to traffic-related particulate pollutants.However, two additional studies independently reported that vitamin D supplementation did not significantly improve the duration of severe asthma attacks [18,32] but significantly reduced the occurrence and exacerbation of asthma [18].Of note, in the present study, asthma was induced by OVA challenge and aggravated by PM exposure, causing an increased count of eosinophils, airway inflammatory cell infiltration, and the upregulation of Th2/Th1 ratio in the lung.Importantly, vitamin D treatment significantly reversed these changes.Interestingly, Rigo et al. [45] reported that vitamin D3 is involved in regulating the innate immune defenses of the airway epithelium, and its deficiency contributes to the exacerbation of OVAinduced airway disease in asthmatic mice [46].Furthermore, another study showed that 1,25-dihydroxyvitamin D3 reduced airway inflammation in asthmatic mice [47].These results confirm that vitamin D3 protects the airways from OVA-induced inflammatory damage.
Vitamin D protects against airway disease through antioxidant and immunomodulatory pathways [48,49].Vitamin D is protective in asthmatics against environmental particulate matter exposure.A cohort study of personal vitamin D status in a predominantly black urban of children shows children with asthma who are vitamin D deficient are more likely to develop sensitivity to PM 2.5 [50].This study reveals that individual vitamin D status in asthmatic children was associated with respiratory symptoms caused by indoor PM 2.5 exposure.Serum 25-OH vitamin D levels below 15.5 ng/mL in asthmatic children increased individual susceptibility to the respiratory effects of indoor PM 2.5 , especially in obese children.In contrast, higher serum 25-OH vitamin D levels were protective against symptoms associated with indoor PM 2.5 exposure.Under exposure to organic dust, pro-inflammatory responses were attenuated in mice fed a highdose vitamin D diet and in cells pretreated with vitamin D compared to mice fed a low-dose vitamin D diet [51].These results suggest that vitamin D supplementation protects against asthma under PM exposure and that this protection is dose-dependent.
Remarkably, in our study, there was a downregulation of HO-1 in the OVA-induced allergic asthma mouse model.As a protective protein, several studies have reported increased HO-1 expression in the airways of asthmatics [52,53].Interestingly, in a mouse model of asthma, HO-1 expression increased in the airway lumen and bronchial submucosa of antigen-challenged mice [54,55].On the contrary, consistent with our findings, additional studies showed that HO-1's expression did not significantly increase in asthmatic mice lung tissues [56,57] and tended to be under-expressed in OVA-stimulated airway epithelial cells [56].During antigen presentation, dendritic cells (DC) cells with high HO-1 expression secrete high levels of IL-10 and TGF-β, and low levels of IL-12 and IL-23.In turn, the differentiation of naive T cells to Treg cells is promoted, and the differentiation of naive T cells to Th2 and Th17 cells is inhibited [58,59], preventing further inflammation.Such observations suggest that high HO-1 expression and anti-inflammatory effects may occur during the initial immune phase.This finding is consistent with our previous study showing that the Th1/Th2 ratio remains relatively constant in OVA-induced mice at the same time, Treg/Th17 changes rapidly, eventually leading to pathological lesions in the respiratory tract [60].However, to confirm and validate this hypothesis, more relevant experiments need to be conducted.
Our study bridges the gap between observational epidemiological data and experimental animal models by investigating the intricate signaling pathways through which vitamin D exerts its protective effects in PMexposed asthma.Through our comprehensive approach, we provide valuable insights into the biological significance of vitamin D and its potential role in mitigating the deleterious impact of particulate matter on asthma.While, Nrf2 maintains cellular redox homeostasis by regulating the downstream antioxidant HO-1 [20,21], thereby providing cytoprotection against oxidative stress-induced cellular damage.In this article, from an animal model, we detected a significant increase in the expression of iNOS in asthmatic lung tissues in response to PM, which was significantly down-regulated after administration of Vit D treatment.Hence we suggest that this pathway also touches a significant oxidative stress response.The process of inflammation was explored in our paper only from the pathway of NGF, however, not much validation was made about the factors related to oxidative stress.For example, the expression of iNOS and ROS and the release of NO under Vit D or PM treatment could be examined in a macrophage cell system or using an airway epithelial cell system.In addition, the reason that the metrics in the Asthma group and the Asthma + PM + Vit D group were not compared in this paper is that the variability of the metrics between the two groups was inconsistent in our results, e.g., the difference in NGF was significant in serum but not in BALF.Therefore, our results do not definitively conclude whether vitamin D can completely alleviate the inflammatory response in asthma due to PM.And to clarify this mechanism, another study is needed, such as the exploration of the concentration gradient of administration and the exploration of the time gradient of administration.Consequently, this research direction is a long way to go, and a lot of basic research is still needed to clarify the inflammatory mechanism of this pathway, and we will conduct experiments in this direction in the future in order to clarify the more specific mechanism of action.

Conclusion
Our study shows that vitamin D is involved, in vivo, in regulating inflammation in PM-exposed asthma.Additionally, we demonstrate that vitamin D plays a protective role, in vitro, in PM-exposed allergic asthma by regulating the Nrf2/HO-1 and NGF signaling pathways.
Our study provides mechanistic evidence to support that vitamin D supplementation can mitigate the deleterious effects of particulate matter on asthma.

Fig. 1
Fig. 1 Vitamin D improves PM-exposed lung histopathological inflammation in asthmatic mice.Thirty-two female mice were randomly assigned to the Control group, Asthma group, Asthma + PM group, or Asthma + PM + Vit D group.(a) Schematic illustration of the mouse modeling process.The gray arrows indicate that each mouse was administered normal saline instead.The blue arrow indicates that each mouse was injected intraperitoneally with OVA sensitization solution.The yellow arrow indicates that the mice were exposed to 1% OVA for 30 min.The green arrow indicates that the mouse was intratracheally instilled with 100ug of PM.The red arrow indicates that the mouse was gavaged with Vit D. (b) Airways resistance (Rrs) and compliance (Crs) of different groups ( # p < 0.01).(c) Airway inflammation (H&E staining, ×200).(d) Inflammatory index.(e) proportion of inflammatory cells in BALF (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001)

Fig. 3
Fig. 3 Vitamin D activates the Nrf2/HO-1 signaling pathway in mouse asthma.(a) qPCR was used to analyze Nrf2, HO-1 and NGF's gene expression levels in lung tissue.(b) immunoblot analysis of the protein expression of Nrf2 and HO-1 in lung tissue.(c) gray-scale values count the relative protein expression of Nrf2 (nucleus) relative to Nrf2 (cytoplasm) and HO-1 relative to β-actin.(d) qPCR analysis of iNOS gene expression level in lung tissue.(e) immunoblot analysis of the protein expression of iNOS in lung tissue.(f) gray-scale values count the relative protein expression of iNOS relative to β-actin.(g) ELISA analysis of the NGF protein expression levels in BALF and serum.(* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001)